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BENEFICIAL EFFECT OF A CONTROLLED CHINESE HERBAL REMEDY
F.Marotta A.Rouge H.Anzulovic M.Harada G.M.Ideo K.Kajikawa N.Yahaihara G.Princess G. Ideo α-Ω Techonolab
2001/10/12-13/
We tested K-17.22 (Yojyo-Henshiko: K-22, Kyotsu Inc., Tokyo, Japan),a controlled herbal “ hepatoprotecitve” formula, in CCL4-induced liver toxicity. Wistar rats were allocated into 3 groups:
A) given a s.c. injection of 0.1ml/100g b.w. of CCL4 in olive oil(1:1 v/v) b.i.d. for 4 weeks;
B) as A, plus 50mg/kg of K-17.22/-5% glucose p.o.;
C) as B but with K-17.22 given 1 week after the first injection of CCL4.
As compared to control, group A showed a significant decrease of GSH>45%, p<0.001) and GSSG(P<0.01) liver content, a lower liver wet weight (p<0.01) together with an increase of transaminases(>15-fold, p<0.001) whereas both groups B and C showed a mild transaminases increase and liver necro-inflammatory score (p<0.05 vs A).
Group A showed an > 30% decrease of Y protein and of GST activity (p<0.01 vs control) which were reverted to normal by K-17.22(p<0.05 vs A). On hepatocyte culture it appeared that concentrations as low as 10 μg/ml of K-17.22 significantly mitigated CCL4 hepatocyte damage (P<0.05) comparably to 100μg /ml sylymarin, while 100μg/ml was more protective than either silymarin 100μg/ml or glycyrrhizin 10μg/ml (p<0.05).
These preliminary data suggest that K-17.22 exerts an highly sparing and prolonged effect (either preventive and therapeutic) on GSH depletion and on the conjugate liver GSH/GSSG redox system in CCL4-induced liver injury.
EFFECT OF A NUTRITIONAL APPROACH WITH K-17.22 ON ENDOGENOUS HEPATIC ANTIOXIDANT SYSTEM.
F.Marotta, M. Harada, N. Yanaihara, G.M. Ideo, MG Seal, CC Wu, P Safran, G.Ideo. Hepato-GI Dept., S.Giuseppe Hosp., Milano, Italy; MCH Hospital, Tokyo, Japan; Yanaihara Institute, Shizuoka, Japan SFJO & Labs., Paris, France; Dept. of Surgery, Vet. Hospital, Taichung, Taiwan;
In the present study we tested a natural compound, i.e. K-17.22, which is endowed by transaminases-lowering effect in HCV patients, on free radicals- related liver damage by ischemia/reperfusion injury.
Wistar rats were fed for 2 weeks with either A) standard diet or B) standard diet added with 30mg of K-17.22 (Yojyo-Henshiko: K-17.22, Kyotsu Inc., Tokyo, Japan).
A classical ischemia/ reperfusion liver model was prepared and after 60 min of reperfusion, hepatic tissue blood flow was measured and the rats were sacrificed. A separate survival study was done as well. The following parameters were checked: liver tissue peroxides, SOD, Catalase, GSH metabolism, hepatic tissue blood flow and radicals-trapping ability of K-17.22 by ESR. After 60 min of reperfusion, B group showed a significantly lower MDA level(p<0.05 vs controls) with an overall impairment of the liver antioxidant defense system(p<0.001). In particular, GSH and GSH-Px reverted to normal with a significantly lower ALT(GPT) level(p<0.05).
K-17.22 didn’t show any direct free radicals-trapping ability either on superoxide nor on hydroxyl radicals systems. Ischemia-reperfusion phenomenon caused a nearly 40% drop of the liver blood flow in A group (p<0.001 vs sham-op.)Pretreatment with K-17.22 enabled a recovery of such haemodynamic parameter(p<0.05 vs untreated group). Only 20% of rats survived after liver ischemia while B group yielded a 45% survival rate(p<0.05). The present nutritional approach seems to offer a noteworthy boosting ability on endogenous free radicals scavengers array which is unrelated to its direct in vitro action.
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